Document Type
Thesis
Publication Date
1992
Disciplines
Biology
Advisor
Marcus Webster
Abstract
Oxygen equilibrium curves of dilute hemoglobin solutions from deer mice (Peromyscus leucopus) and meadow voles (Microtus pennsylvanicus) were determined by optical tonometry at pH 6.8 and pH 7.4. For deer mouse hemoglobin, P50 6.8 = 4.54 KPa, n7.4 (cooperativity index at pH 7.4) = 1.13, n6.8 = 1.60 and Bohr shift = 1.91. For meadow vole hemoglobin P50 7.4 = 2.74 KPa, P50 6.8 = 6.16 KPa, n7.4 = 1.11, n6.8 = 1.63, and Bohr shift = 2.35. The method was evaluated for use in the undergraduate laboratory.
Copyright Statement
Available by permission of the author. Reproduction or retransmission of this material in any form is prohibited without expressed written permission of the author.
Recommended Citation
Loeffelholz, James, "A comparative Study of the Oxygen Dissociation Curves of Deer Mouse (Peromyscus leucopus) and Meadow Vole (Microtus pennsylvanicus) Hemoglobin" (1992). Honors Theses, 1963-2015. 318.
https://digitalcommons.csbsju.edu/honors_theses/318