Oxygen equilibrium curves of dilute hemoglobin solutions from deer mice (Peromyscus leucopus) and meadow voles (Microtus pennsylvanicus) were determined by optical tonometry at pH 6.8 and pH 7.4. For deer mouse hemoglobin, P50 6.8 = 4.54 KPa, n7.4 (cooperativity index at pH 7.4) = 1.13, n6.8 = 1.60 and Bohr shift = 1.91. For meadow vole hemoglobin P50 7.4 = 2.74 KPa, P50 6.8 = 6.16 KPa, n7.4 = 1.11, n6.8 = 1.63, and Bohr shift = 2.35. The method was evaluated for use in the undergraduate laboratory.
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Loeffelholz, James, "A comparative Study of the Oxygen Dissociation Curves of Deer Mouse (Peromyscus leucopus) and Meadow Vole (Microtus pennsylvanicus) Hemoglobin" (1992). Honors Theses. 318.