Document Type


Publication Date

Spring 2016


Biochemistry, Biophysics, and Structural Biology | Biology | Life Sciences


David Mitchell, Biology


Escherichia coli use genetic mutations to rapidly adapt to stressful situations. This study found that growing E. coli in increasing concentrations of streptomycin resulted in an increase in the strain's minimum inhibitory concentration (MIC) of streptomycin, a measurement of antibiotic resistance. Sequential exposure to streptomycin and nalidixic acid resulted in a strain that had acquired resistance to an antibiotic from two antibiotic classes. Genetic sequencing indicated a mutation in the 30S ribosomal subunit protein s12 gene concurrent with streptomycin resistance. Additionally, point mutations in helicase and fimbrial protein FimH genes are implicated in nalidixic acid resistance.

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