Like mother, like daughter? An analysis of proteins expressed by wild type and mutant phenotypes of Candida albicans using SDS-PAGE
The dimorphic fungus Candida albicans causes both surface and systemic infections depending on its morphology. The ability to switch between a yeast and mycelia form plays a significant role in the organism’s virulence. Dr. David Soll and his students showed that Candida albicans has a second mechanism of generating phenotypic variability which he called white-opaque phenotypic switching. Both white and opaque forms are fully dimorphic. Though dry weight, titer, and Bradford Assay, quantitative amounts of protein among the wild type strains (WO-1 opaque, WO-1 white, L26 smooth/opaque, L26 wrinkly/white) and CSB/SJU series mutants (A-72 03EU p7-1, A-72 03EU p7-5, and M246 op 4/10) were measured. SDS-PAGE was used to compare several potential cell lysis methods and to separate cellular proteins.
Wilant, Laura, "Like mother, like daughter? An analysis of proteins expressed by wild type and mutant phenotypes of Candida albicans using SDS-PAGE" (2006). Honors Theses. 299.