Like mother, like daughter? An analysis of proteins expressed by wild type and mutant phenotypes of Candida albicans using SDS-PAGE

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The dimorphic fungus Candida albicans causes both surface and systemic infections depending on its morphology. The ability to switch between a yeast and mycelia form plays a significant role in the organism’s virulence. Dr. David Soll and his students showed that Candida albicans has a second mechanism of generating phenotypic variability which he called white-opaque phenotypic switching. Both white and opaque forms are fully dimorphic. Though dry weight, titer, and Bradford Assay, quantitative amounts of protein among the wild type strains (WO-1 opaque, WO-1 white, L26 smooth/opaque, L26 wrinkly/white) and CSB/SJU series mutants (A-72 03EU p7-1, A-72 03EU p7-5, and M246 op 4/10) were measured. SDS-PAGE was used to compare several potential cell lysis methods and to separate cellular proteins.