Document Type
Article
Publication Date
8-25-2023
Disciplines
Biology | Genetics and Genomics
Abstract
Anterior chamber depth (ACD) is a quantitative trait associated with primary angle closure glaucoma (PACG). Although ACD is highly heritable, known genetic variations explain a small fraction of the phenotypic variability. The purpose of this study was to identify additional ACD-influencing loci using strains of mice. Cohorts of 86 N2 and 111 F2 mice were generated from crosses between recombinant inbred BXD24/TyJ and wild-derived CAST/EiJ mice. Using anterior chamber optical coherence tomography, mice were phenotyped at 10–12 weeks of age, genotyped based on 93 genome-wide SNPs, and subjected to quantitative trait locus (QTL) analysis. In an analysis of ACD among all mice, six loci passed the significance threshold of p = 0.05 and persisted after multiple regression analysis. These were on chromosomes 6, 7, 11, 12, 15 and 17 (named Acdq6, Acdq7, Acdq11, Acdq12, Acdq15, and Acdq17, respectively). Our findings demonstrate a quantitative multi-genic pattern of ACD inheritance in mice and identify six previously unrecognized ACD-influencing loci. We have taken a unique approach to studying the anterior chamber depth phenotype by using mice as genetic tool to examine this continuously distributed trait.
Copyright Statement
Copyright © 2023 Larson et al.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
Larson DR, Kimber AJ, Meyer KJ, Anderson MG. 2023. Anterior chamber depth in mice is controlled by several quantitative trait loci. PLoS ONE 18(8): e0286897. https://doi.org/10.1371/journal.pone.0286897