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Rachel Marston, English


The purpose of this experiment was to analyze and determine the morphological differences and behavioral abnormalities that arise in third-instar Drosophila melanogaster larvae in response to bisphenol-A exposure. Drosophila melanogaster from genetic strains that induce motor neuron GFP expression were used: OK371-GAL4, W*;; UAS-GFP (1010T10), and cd8-GFP; D48 GAL4 GAL80. Drosophila were exposed to one of three conditions: control, .44 mM, and 4.4 mM concentrations of bisphenol-A (BPA) during embryonic and larval development. The larvae raised were dissected, fixed, stained using immunohistochemistry in order to observe the difference in their motor nerve morphology after BPA exposure. Speciments were viewed on an inverted fluorescent microscope in order to measure the qualitative appearance and surface area of the neuro muscular junction between muscles 6 and 7. Results are important for understanding effects of BPA exposure on patterning and development of nervous system.