Title

The Use of the DNA Comet Assay to Detect Genetic Damage in Rana pipiens Taken from Affected Versus Non-Affected Sites

Document Type

Thesis

Publication Date

1999

Advisor

Jeanne Marie Lust, OSB

Abstract

Deformed frogs have been appearing frequently in Minnesota and throughout the United States. One hypothesis for the cause of deformities is genetic damage as a result of pesticides. This project will use the DNA Comet Assay to determine the extent of genetic damage in affected sites (sites with a greater number of deformities) versus non-affected sites (sites with fewer deformities). The DNA Comet Assay involves electrophoresis of cells in which the membranes have been solubilized and the DNA uncoiled. Electrophoresis spreads the genetic material. The length of the spread indicates the amount of genetic damage in the form of single and double strand breaks. The results indicate a difference in frogs collected from affected sites and non-affected sites.

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