Edward J. McIntee, Chemistry; Henry V. Jakubowski, Chemistry
The two active isoforms of Low Molecular Weight Protein Tyrosine Phosphatase (LMW-PTP) have inverse cellular effects on cancer. Isoform A has been found to be anti-oncogenic, while overexpression of Isoform B is oncogenic. Inhibitors of Isoform B could have anti-cancer effects, however, it is of utmost importance that these inhibitors exhibit specificity for Isoform B and do not affect the function of Isoform A. Isoform A was recombinantly expressed, purified and characterized in order to compare its structure and function to Isoform B. It is hoped that this characterization, along with the synthesis and screening of potential inhibitors for both isoforms, will streamline the discovery and optimization of new anti-cancer candidates that specifically target LMW-PTP Isoform B.
Sinner, Erica K., "Determination of Inhibitor Specificity for Low Molecular Weight Protein Tyrosine Phosphatase Isoforms A and B" (2015). Honors Theses. 67.