Title

Sensitivity of a S. cerevisiae deletion mutant to DNA-damaging agents and in vivo complementation by the human FEN-1 protein

Authors

Ryan J. Hansen, College of Saint Benedict/Saint John's University
Errol C. Friedberg
Michael S. Reagan, College of Saint Benedict/Saint John's UniversityFollow

Document Type

Article

Publication Date

11-2000

Disciplines

Biology | Genomics

Abstract

We have investigated the sensitivity to DNA-damaging agents of a strain of Saccharomyces cerevisiae containing a deletion of the RAD27 gene. The mutant strain is sensitive to a number of alkylating agents that modify DNA at a variety of positions, including one that produces primarily phosphotriesters. In contrast, the mutant strain is not sensitive to the oxidizing agent hydrogen peroxide. The introduction of a plasmid containing the FEN-1 gene (the human ortholog of the RAD27 gene) can substantially complement the sensitivity to alkylating agents observed in the mutant strain.

Recommended Citation

Hansen RJ, Friedberg EC, Reagan MS. 2000. Sensitivity of a S. cerevisiae deletion mutant to DNA-damaging agents and in vivo complementation by the human FEN-1 protein. Mutation Research 461(3): 243-248.